{batsch}
provides real-time PCR data sets by Batsch et al. (2008) in tidy format. There are five data sets bundled with this package, one for each PCR target:
SLC6A14r
for rat SLC6A14SLC22A13h
for human SLC22A13EMTp
for pig EMTETTch
for chicken ETTGAPDHh
for human GAPDHEach data set comprises a five-point, four-fold dilution series. For each concentration there are three replicates. Each amplification curve is 45 cycles long.
Install {batsch}
from CRAN:
You can instead install the development version of {batsch}
from GitHub:
library(batsch)
SLC6A14r |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = interaction(dilution, replicate),
col = as.factor(dilution)
)) +
geom_line(linewidth = 0.5) +
labs(y = "Raw fluorescence", colour = "Dilution factor", title = "Rat SLC6A14") +
guides(color = guide_legend(override.aes = list(size = 0.5)))
SLC22A13h |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = interaction(dilution, replicate),
col = as.factor(dilution)
)) +
geom_line(linewidth = 0.5) +
labs(y = "Raw fluorescence", colour = "Dilution factor", title = "Human SLC22A13") +
guides(color = guide_legend(override.aes = list(size = 0.5)))
EMTp |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = interaction(dilution, replicate),
col = as.factor(dilution)
)) +
geom_line(linewidth = 0.5) +
labs(y = "Raw fluorescence", colour = "Dilution factor", title = "Pig EMT") +
guides(color = guide_legend(override.aes = list(size = 0.5)))
ETTch |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = interaction(dilution, replicate),
col = as.factor(dilution)
)) +
geom_line(linewidth = 0.5) +
labs(y = "Raw fluorescence", colour = "Dilution factor", title = "Chicken ETT") +
guides(color = guide_legend(override.aes = list(size = 0.5)))
GAPDHh |>
ggplot(mapping = aes(
x = cycle,
y = fluor,
group = interaction(dilution, replicate),
col = as.factor(dilution)
)) +
geom_line(linewidth = 0.5) +
labs(y = "Raw fluorescence", colour = "Dilution factor", title = "Human GAPDH") +
guides(color = guide_legend(override.aes = list(size = 0.5)))
Please note that the {batsch}
project is released with a Contributor Code of Conduct. By contributing to this project, you agree to abide by its terms.